Article
Increased sample capacity for genotyping and expression profiling by kinetic polymerase chain reaction.
Analytical biochemistry - 1 Jun 2004
Watson Robert M, Griaznova Olga I, Long Christopher M, Holland Michael J
Abstract excerpt
We fabricated and evaluated high-throughput kinetic thermal cyclers with 768-reaction capacity for kinetic polymerase chain reaction (kPCR)-based genotyping and kinetic reverse transcription (kRT)-PCR-based transcript quantitation. The system uses dye-based detection with ethidium bromide and a single DNA polymerase-based PCR or RT-PCR assay. Allele-specific detection of the two most common hereditary...
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