Article
Transcript quantitation in total yeast cellular RNA using kinetic PCR.
Nucleic acids research - 15 Jan 2000
Kang J J, Watson R M, Fisher M E, Higuchi R, Gelfand D H, Holland M J
Abstract excerpt
Kinetically monitored, reverse transcriptase-initiated PCR (kinetic RT-PCR, kRT-PCR) is a novel application of kinetic PCR for high throughput transcript quantitation in total cellular RNA. The assay offers the simplicity and flexibility of an enzyme assay with distinct advantages over DNA microarray hybridization and SAGE technologies for certain applications. The reproducibility, sensitivity and accuracy of the...
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