Article
Two-temperature LATE-PCR endpoint genotyping.
BMC biotechnology - 4 Dec 2006
Sanchez J Aquiles, Abramowitz Jessica D, Salk Jesse J, Reis Arthur H, Rice John E, Pierce Kenneth E, Wangh Lawrence J
Abstract excerpt
BACKGROUND: In conventional PCR, total amplicon yield becomes independent of starting template number as amplification reaches plateau and varies significantly among replicate reactions. This paper describes a strategy for reconfiguring PCR so that the signal intensity of a single fluorescent detection probe after PCR thermal cycling reflects genomic composition. The resulting method corrects for product yield...
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