Article
SNP genotyping using single-tube fluorescent bidirectional PCR.
BioTechniques - 1 Jul 2002
Waterfall Christy M, Cobb Benjamin D
Abstract excerpt
SNP genotyping is a well-populatedfield with a large number of assay formats offering accurate allelic discrimination. However, there remains a discord between the ultimate goal of rapid, inexpensive assays that do not require complex design considerations and involved optimization strategies. We describe the first integration of bidirectional allele-specific amplification, SYBR Green I, and rapid-cycle PCR to...
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