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Article

Automated DNA extraction, quantification, dilution, and PCR preparation for genotyping by high-resolution melting.

Journal of biomolecular techniques : JBT - 1 Dec 2010

Seipp Michael T, Herrmann Mark, Wittwer Carl T

Abstract excerpt

Genotyping by high-resolution amplicon melting uses only two PCR primers per locus and a generic, saturating DNA dye that detects heteroduplexes as well as homoduplexes. Heterozygous genotypes have a characteristic melting curve shape and a broader width than homozygous genotypes, which are usual...

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PMID: 21119928
PMCID: PMC2977966

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 2 Sept 2026. Prepared record publication-21119928-2026-09-02.2. Manifest publication-graph-cohort-2026-09-02.2.

Source receipt: PubMed via Helena Literature Corpus, retrieved 2 Sept 2026.

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