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High-Throughput Genotyping by CD-PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2025

Nakanishi Yoichi, Taga Shunta

Abstract excerpt

The process of extracting genomic DNA from biological samples is a bottleneck in PCR genotyping. Here, we describe a speedy genotyping method that combines simple genomic DNA extraction using hot alkaline/SDS and direct use of the extract in an SDS-tolerant cyclodextrin-PCR (CD-PCR).

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PMID: 40580288
DOI: 10.1007/978-1-0716-4642-7_8

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