Article
The LightTyper: high-throughput genotyping using fluorescent melting curve analysis.
BioTechniques - 1 Jun 2003
Bennett C D, Campbell M N, Cook C J, Eyre D J, Nay L M, Nielsen D R, Rasmussen R P, Bernard P S
Abstract excerpt
Instrumentation, chemistry, and software for high-throughput genotyping using fluorescent melting curves are described. The LightTyper system provides post-amplification genotyping within 10 min using samples in 96- or 384-well microplate formats. The system is homogenous because all reagents are added at the beginning of the reaction and there is no sample manipulation between amplification and genotyping....
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