Article
Linear-after-the-exponential (LATE)-PCR: an advanced method of asymmetric PCR and its uses in quantitative real-time analysis.
Proceedings of the National Academy of Sciences of the United States of America - 17 Feb 2004
Sanchez J Aquiles, Pierce Kenneth E, Rice John E, Wangh Lawrence J
Abstract excerpt
Conventional asymmetric PCR is inefficient and difficult to optimize because limiting the concentration of one primer lowers its melting temperature below the reaction annealing temperature. Linear-After-The-Exponential (LATE)-PCR describes a new paradigm for primer design that renders assays as efficient as symmetric PCR assays, regardless of primer ratio. LATE-PCR generates single-stranded products with...
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