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Article

Straightforward detection of SNPs in double-stranded DNA by using exonuclease III/nuclease S1/PNA system.

Nucleic acids research - 24 Feb 2004

Ren Binzhi, Zhou Jing-Min, Komiyama Makoto

Abstract excerpt

Single-nucleotide polymorphisms (SNPs) in double-stranded DNA (dsDNA) have been straightforwardly genotyped by matrix-assisted laser desorption/ ionization time-of-flight mass spectrometry (MALDI-TOF MS). Peptide nucleic acid (PNA), a DNA analog, was used as a probe molecule. In its presence, genomic dsDNA was first treated with exonuclease III and then with nuclease S1. By these one-pot reactions,...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Identifiers

PMID: 14982961
DOI: 10.1093/nar/gnh039
PMCID: PMC390314

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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