Article
Simultaneous detection of multiple single nucleotide polymorphism by single-strand-specific nuclease and PNA probe.
Nucleic acids research. Supplement (2001) - 1 Jan 2003
Ye Sheng, Liang Xingguo, Yamamoto Yoji, Zhou Jing-Min, Tomita Takafumi, Aburatani Hiroyuki, Komiyama Makoto
Abstract excerpt
The combination of PNA (peptide nucleic acid) and single-strand-specific nuclease have been used for detection of single nucleotide polymorphisms (SNPs). When DNA is perfectly complementary to PNA, it is protected from digestion by the nuclease. If there exists a single-base mismatch between them, however, the DNA is completely digested. These differences are visualized by using 3,3'-diethylthiadicarbocyanine...
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