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Article

Conditional protein alleles using knockin mice and a chemical inducer of dimerization.

Molecular cell - 1 Dec 2003

Stankunas Kryn, Bayle J Henri, Gestwicki Jason E, Lin Yun-Ming, Wandless Thomas J, Crabtree Gerald R

Abstract excerpt

We have developed a general method of making conditional alleles that allows the rapid and reversible regulation of specific proteins. A mouse line was produced in which proteins encoded by the endogenous glycogen synthase kinase-3 beta (GSK-3beta) gene are fused to an 89 amino acid tag, FRB*. FRB* causes the destabilization of GSK-3beta, producing a severe loss-of-function allele. In the presence of C20-MaRap, a...

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Identifiers

PMID: 14690613
DOI: 10.1016/s1097-2765(03)00491-x

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 6 Sept 2026. Manifest publication-graph-cohort-2026-09-06.1.

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