Article
Caution! Analyze transcripts from conditional knockout alleles.
Transgenic research - 1 Jun 2009
Yang Shao H, Bergo Martin O, Farber Emily, Qiao Xin, Fong Loren G, Young Stephen G
Abstract excerpt
A common strategy for conditional knockout alleles is to "flox" (flank with loxP sites) a 5' exon within the target gene. Typically, the floxed exon does not contain a unit number of codons so that the Cre-mediated recombination event yields a frameshift and a null allele. Documenting recombination within the genomic DNA is often regarded as sufficient proof of a frameshift, and the analysis of transcripts is...
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