Article
Generation of a germ cell nuclear factor conditional allele in mice.
Genesis (New York, N.Y. : 2000) - 1 Dec 2003
Lan Zi-Jian, Xu Xueping, Cooney Austin J
Abstract excerpt
Two recombination steps in embryonic stem (ES) cells were adopted to generate a floxed Germ Cell Nuclear Factor (GCNF) allele. First, a targeting vector containing a loxP site upstream of exon 4, encoding the DNA binding domain (DBD), and a floxed NeoTK double selection cassette downstream of exon 4 was integrated into the GCNF locus by homologous recombination. Second, a Cre-expressing vector was transiently...
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