Identification of a novel promoter in the replication control region of plasmid R6K.

doi:10.1128/jb.174.14.4777-4782.1992

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Back to search

Article

Identification of a novel promoter in the replication control region of plasmid R6K.

Journal of bacteriology - 1 Jul 1992

Mukerji P, Greener A, Filutowicz M

Abstract excerpt

A novel source of transcription has been detected in the replication region of plasmid R6K by using fusions involving the galK reporter gene. The -35 and -10 consensus RNA polymerase binding sites were identified in the region overlapping the binding sites for the R6K-encoded replication protein pi. Transcription from this promoter, designated P2, is repressed in vivo by pi-protein levels that are inhibitory for...

Topics

Bacterial Proteins
Base Sequence
Binding Sites
Carrier Proteins
DNA Replication
Escherichia coli
Escherichia coli Proteins
Gene Expression Regulation, Bacterial
Lipoproteins
Molecular Sequence Data
Mutagenesis

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Mutation

Operon

Plasmids

Promoter Regions, Genetic

Recombinant Fusion Proteins

Transcription, Genetic

Source

Open in PubMed Open full text

Identifiers

PMID: 1624464
DOI: 10.1128/jb.174.14.4777-4782.1992
PMCID: PMC206275

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1624464-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification of a novel promoter in the replication control region of plasmid R6K.PMID 1624464Read ↗

Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Semantic proximity Read ↗

Autoregulation-deficient mutant of the plasmid R6K-encoded pi protein distinguishes between palindromic and nonpalindromic binding sites.Semantic proximity Read ↗

Biochemical investigations of control of replication initiation of plasmid R6K.Semantic proximity Read ↗

Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.Semantic proximity Read ↗

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.Semantic proximity Read ↗

N-terminal truncated forms of the bifunctional pi initiation protein express negative activity on plasmid R6K replication.Semantic proximity Read ↗

Reconstitution of R6K DNA replication in vitro using 22 purified proteins.Semantic proximity Read ↗

Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance.Semantic proximity Read ↗

Transcription-induced deletions in plasmid vectors: M13 DNA replication as a source of instability.Semantic proximity Read ↗

Replication initiation at a distance: determination of the cis- and trans-acting elements of replication origin alpha of plasmid R6K.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.