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Article

Identification of a novel promoter in the replication control region of plasmid R6K.

Journal of bacteriology - 1 Jul 1992

Mukerji P, Greener A, Filutowicz M

Abstract excerpt

A novel source of transcription has been detected in the replication region of plasmid R6K by using fusions involving the galK reporter gene. The -35 and -10 consensus RNA polymerase binding sites were identified in the region overlapping the binding sites for the R6K-encoded replication protein pi. Transcription from this promoter, designated P2, is repressed in vivo by pi-protein levels that are inhibitory for...

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Identifiers

PMID: 1624464
DOI: 10.1128/jb.174.14.4777-4782.1992
PMCID: PMC206275

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

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Research trail→

Click a neighbor to travelStep 1 · 12 closest

Identification of a novel promoter in the replication control region of plasmid R6K.PMID 1624464Read ↗

Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.Semantic proximity Read ↗

Autoregulation-deficient mutant of the plasmid R6K-encoded pi protein distinguishes between palindromic and nonpalindromic binding sites.Semantic proximity Read ↗

Biochemical investigations of control of replication initiation of plasmid R6K.Semantic proximity Read ↗

Roles of a 106-bp origin enhancer and Escherichia coli DnaA protein in replication of plasmid R6K.Semantic proximity Read ↗

Replication of the R6K gamma origin in vitro: dependence on wt pi and hyperactive piS87N protein variant.Semantic proximity Read ↗

N-terminal truncated forms of the bifunctional pi initiation protein express negative activity on plasmid R6K replication.Semantic proximity Read ↗

Reconstitution of R6K DNA replication in vitro using 22 purified proteins.Semantic proximity Read ↗

Activation of distant replication origins in vivo by DNA looping as revealed by a novel mutant form of an initiator protein defective in cooperativity at a distance.Semantic proximity Read ↗

Transcription-induced deletions in plasmid vectors: M13 DNA replication as a source of instability.Semantic proximity Read ↗

Replication initiation at a distance: determination of the cis- and trans-acting elements of replication origin alpha of plasmid R6K.Semantic proximity Read ↗

Mutations in the gene encoding the replication-initiation protein of plasmid RK2 produce elevated copy numbers of RK2 derivatives in Escherichia coli and distantly related bacteria.Semantic proximity Read ↗

Copy-up mutants of the plasmid RK2 replication initiation protein are defective in coupling RK2 replication origins.Semantic proximity Read ↗

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