Article
N-terminal truncated forms of the bifunctional pi initiation protein express negative activity on plasmid R6K replication.
Molecular & general genetics : MGG - 1 Oct 1990
Greener A, Filutowicz M S, McEachern M J, Helinski D R
Abstract excerpt
The replication initiation protein pi of the Escherichia coli plasmid R6K is a dual regulator in the control of plasmid copy number, functioning both as a specific initiator and inhibitor of replication. While the biochemical basis of these activities is not known, initiator activity requires binding of the protein to the seven 22 bp direct repeats within the gamma-origin region. By deleting C-terminal segments...
Topics
- Amino Acid Sequence
- Cloning, Molecular
- DNA Helicases
- DNA Replication
- DNA, Bacterial
- DNA-Binding Proteins
- Escherichia coli
- Molecular Sequence Data
- Mutation
- Peptide Initiation Factors
- R Factors