Article
Altered (copy-up) forms of initiator protein pi suppress the point mutations inactivating the gamma origin of plasmid R6K.
Journal of bacteriology - 1 Dec 1995
Urh M, Flashner Y, Shafferman A, Filutowicz M
Abstract excerpt
The R6K gamma origin core contains the P2 promoter, whose -10 and -35 hexamers overlap two of the seven binding sites for the R6K-encoded pi protein. Two mutations, P2-201 and P2-203, which lie within the -35 region of P2, are shown to confer a promoter-down phenotype. We demonstrate here that th...
Topics
- Bacterial Proteins
- Base Sequence
- DNA Helicases
- DNA Replication
- DNA-Binding Proteins
- Escherichia coli
- Genetic Variation
- Molecular Sequence Data
- Mutagenesis, Site-Directed
- Peptide Initiation Factors
- Point Mutation