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Article

Mapping dominant-negative mutations of anthrax protective antigen by scanning mutagenesis.

Proceedings of the National Academy of Sciences of the United States of America - 25 Nov 2003

Mourez Michael, Yan Ming, Lacy D Borden, Dillon Lisa, Bentsen Lori, Marpoe Amy, Maurin Clémence, Hotze Eileen, Wigelsworth Darran, Pimental Ruth-Anne, Ballard Jimmy D, Collier R John, Tweten Rodney K

Abstract excerpt

The protective antigen (PA) moiety of anthrax toxin transports edema factor and lethal factor to the cytosol of mammalian cells by a mechanism that depends on its ability to oligomerize and form pores in the endosomal membrane. Previously, some mutated forms of PA, designated dominant negative (DN), were found to coassemble with wild-type PA and generate defective heptameric pore-precursors (prepores). Prepores...

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Methodology Data sources Editorial policy Privacy Delete account & data About Contact Helena Bioinformatics

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Identifiers

PMID: 14623961
DOI: 10.1073/pnas.2436299100
PMCID: PMC283502

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Source receipt: pubmed, retrieved 7 Sept 2026.

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