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Article

Characterization and molecular modeling of a highly stable anti-Hepatitis B surface antigen scFv.

Molecular immunology - 1 Dec 2003

Bose Biplab, Chugh Dipti A, Kala Mrinalini, Acharya Subrat K, Khanna Navin, Sinha Subrata

Abstract excerpt

We raised a mouse monoclonal antibody (5S) against the 'a' epitope of the Hepatitis B surface antigen (HBsAg) by selecting for binding of the hybridoma supernatant in conditions that usually destabilize protein-protein interactions. This antibody, which was protective in an in vitro assay, had a high affinity with a relative dissociation constant in the nanomolar range. It also displayed stable binding to antigen...

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Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

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Source

Identifiers

PMID: 14597165
DOI: 10.1016/j.molimm.2003.07.002

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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