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Solubility, Stability, and Avidity of Recombinant Antibody Fragments Expressed in Microorganisms

25 Sept 2020

Abstract excerpt

Solubility of recombinant proteins (i.e., the extent of soluble versus insoluble expression in heterogeneous hosts) is the first checkpoint criterion for determining recombinant protein quality. However, even soluble proteins often fail to represent functional activity because of the involvement of nonfunctional, misfolded, soluble aggregates, which compromise recombinant protein quality. Therefore, screening of...

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PMID: 33101218
DOI: 10.3389/fmicb.2020.01927

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