Article
Improved antigen binding by a CD20-specific single-chain antibody fragment with a mutation in CDRH1.
Molecular immunology - 1 Feb 2006
Adamson P J, Millard D J, Hohmann A W, Mavrangelos C, Macardle P J, Pilkington G, Mulhern T D, Tedder T F, Zola H, Nicholson I C
Abstract excerpt
We have prepared single-chain immunoglobulin Fv fragments from the CD20-specific hybridoma HB13d. One scFv clone demonstrated strong binding to a CD20-derived peptide by ELISA and to CD20-positive cells by flow cytometry, a second had reduced binding, and a third clone did not bind the target antigen. Sequence analysis showed that all three constructs contained shared and unique amino acid changes when compared...
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