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Article

VL position 34 is a key determinant for the engineering of stable antibodies with fast dissociation rates.

Protein engineering - 1 May 2003

Hugo N, Weidenhaupt M, Beukes M, Xu B, Janson J-C, Vernet T, Altschuh D

Abstract excerpt

Predictive engineering of antibodies exhibiting fast kinetic properties could provide reagents for biotechnological applications such as continuous monitoring of compounds or affinity chromatography. Based on covariance analysis of murine germline antibody variable domains, we selected position L34 (Kabat numbering) for mutational studies. This position is located at the VL/VH interface, at the base of the...

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PMID: 12826730
DOI: 10.1093/protein/gzg042

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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