Article
Overexpression of human DNA repair protein N-methylpurine-DNA glycosylase results in the increased removal of N-methylpurines in DNA without a concomitant increase in resistance to alkylating agents in Chinese hamster ovary cells.
Carcinogenesis - 1 Nov 1992
Ibeanu G, Hartenstein B, Dunn W C, Chang L Y, Hofmann E, Coquerelle T, Mitra S, Kaina B
Abstract excerpt
N-Alkylpurines induced in DNA by simple monofunctional alkylating agents are known to be cytotoxic and possibly indirectly mutagenic. These adducts are removed by the ubiquitous N-methylpurine-DNA glycosylase (MPG) in a multistep repair pathway. Chinese hamster ovary (CHO) cell clones expressing 2- to 16-fold enhanced levels of MPG activity were isolated from cells stably transfected with human MPG cDNA...
Topics
- Alkylating Agents
- Amino Acid Sequence
- Animals
- Base Sequence
- CHO Cells
- Cricetinae
- DNA
- DNA Glycosylases
- DNA Repair
- Drug Resistance