Article
Expression of the E. coli fpg protein in CHO cells lowers endogenous oxypurine clustered damage levels and decreases accumulation of endogenous Hprt mutations.
Environmental and molecular mutagenesis - 1 Jun 2006
Paul Sunirmal, Gros Laurent, Laval Jacques, Sutherland Betsy M
Abstract excerpt
Endogenous DNA damage clusters--two or more oxidized bases, abasic sites, or strand breaks within about 20 base pairs on opposing strands--can accumulate in unirradiated mammalian cells, and may be significant origins of spontaneous detrimental biological effects. Factors determining the levels of such endogenous clusters are largely unknown. To determine if cellular repair genotype can affect endogenous cluster...
Topics
- Animals
- Azaguanine
- CHO Cells
- Cricetinae
- Cricetulus
- DNA Damage
- DNA Repair
- DNA-Formamidopyrimidine Glycosylase
- Escherichia coli Proteins
- Hypoxanthine Phosphoribosyltransferase