Article
Increased removal of 3-alkyladenine reduces the frequencies of hprt mutations induced by methyl- and ethylmethanesulfonate in Chinese hamster fibroblast cells.
Nucleic acids research - 11 May 1994
Klungland A, Bjørås M, Hoff E, Seeberg E
Abstract excerpt
We have previously reported the isolation of mammalian cell lines expressing the 3-methyladenine DNA glycosylase I (tag) gene from E. coli. These cells are 2-5 fold more resistant to the toxic effects of methylating agents than normal cells (15). Kinetic measurements of 3-methyladenine removal fr...
Topics
- Adenine
- Alkylation
- Animals
- Blotting, Southern
- CHO Cells
- Cricetinae
- Cricetulus
- DNA Glycosylases
- Escherichia coli
- Ethyl Methanesulfonate
- Hypoxanthine Phosphoribosyltransferase
- Kinetics
- Methyl Methanesulfonate
- Mutation
- N-Glycosyl Hydrolases