Article
DNA repair pathways in mammalian cells analyzed by isolation of ACNU-sensitive Chinese hamster ovary cells.
The Tohoku journal of experimental medicine - 1 Oct 1992
Numata M, Hata H, Tohda H, Yasui A, Oikawa A
Abstract excerpt
1-[(4-amino-2-methyl-5-pyrimidinyl)methyl]-3-(2-chloroethyl)-3- nitrosourea hydrochloride (ACNU) causes chloroethylation of DNA strand followed by cross linking through an ethylene bridge. We recently isolated two ACNU sensitive mutants from mutagenized Chinese hamster ovary cells, and found them to be new drug sensitive recessive mutants (Hata et al. 1991). The O6-methyl guanine DNA methyl transferase (MT)...
Topics
- Animals
- CHO Cells
- Cell Separation
- Cricetinae
- DNA Repair
- Drug Screening Assays, Antitumor
- Mutation
- Nimustine
- Ultraviolet Rays