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Article

Transgenic Chinese hamster V79 cell lines which exhibit variable levels of gpt mutagenesis.

Environmental and molecular mutagenesis - 1 Jan 1990

Klein C B, Rossman T G

Abstract excerpt

The Escherichia coli gpt gene coding for xanthine-guanine phosphoribosyl transferase has been stably transfected into HPRT- Chinese hamster V79 cells. Several gpt- cell lines have been established, which retain the sequence(s) even after long-term culture without selection for gpt. Each cell line exhibits a characteristic spontaneous mutation frequency (10(-5) to 10(-2)) in 6-thioguanine (6TG) selection. While...

Topics

Animals
Blotting, Southern
Cell Line
Cricetinae
Escherichia coli
Genes, Bacterial
Hypoxanthine Phosphoribosyltransferase
Methylnitronitrosoguanidine
Mutagenicity Tests
Mutation
Pentosyltransferases

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Propiolactone

Transfection

Ultraviolet Rays

X-Rays

Source

Identifiers

PMID: 2197084
DOI: 10.1002/em.2850160102

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-2197084-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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