Article
An abasic site analogue activates a c-Ha-ras gene by a point mutation at modified and adjacent positions.
Nucleic acids research - 11 Sept 1992
Kamiya H, Suzuki M, Komatsu Y, Miura H, Kikuchi K, Sakaguchi T, Murata N, Masutani C, Hanaoka F, Ohtsuka E
Abstract excerpt
Synthetic c-Ha-ras genes with an analogue of an abasic site in the first or the second position of codon 12, or in the second position of codon 61 were constructed and transfected into NIH3T3 cells. The genes with the lesions in codon 12 exhibited more focus formation than a normal c-Ha-ras gene, while the gene with the lesion in codon 61 did not. Transformed cells were isolated from the foci, and the c-Ha-ras...
Read the complete abstract on PubMedTopics
Share this publication in a Topic to start or enrich a Post.