Article
c-Ha-ras containing 8-hydroxyguanine at codon 12 induces point mutations at the modified and adjacent positions.
Cancer research - 15 Jun 1992
Kamiya H, Miura K, Ishikawa H, Inoue H, Nishimura S, Ohtsuka E
Abstract excerpt
To determine the type of mutation induced by 8-hydroxyguanine in a mammalian system, we examined the mutations induced by a synthetic c-Ha-ras protooncogene containing 8-hydroxyguanine in the second position of codon 12 (GGC) in NIH3T3 cells. Transfection of this gene significantly increased the number of transformed foci. The c-Ha-ras gene present in these foci was analyzed by the polymerase chain...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Cell Transformation, Neoplastic
- Codon
- DNA Mutational Analysis
- Genes, ras
- Guanine
- Mice
- Molecular Sequence Data
- Mutation