O6-ethylguanine and O6-benzylguanine incorporated site-specifically in codon 12 of the rat H-ras gene induce semi-targeted as well as targeted mutations in Rat4 cells.

doi:10.1093/carcin/17.4.849

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Article

O6-ethylguanine and O6-benzylguanine incorporated site-specifically in codon 12 of the rat H-ras gene induce semi-targeted as well as targeted mutations in Rat4 cells.

Carcinogenesis - 1 Apr 1996

Bishop R E, Pauly G T, Moschel R C

Abstract excerpt

To examine the miscoding properties of modified guanine residues bearing increasingly bulky O6-substituents, Rat4 cells, grown in the presence of O6-benzylguanine to deplete the DNA repair protein O6-alkylguanine-DNA alkyltransferase, were transfected with plasmids carrying H-ras genes in which O...

Topics

Animals
Base Sequence
Cell Line
Cell Line, Transformed
Clone Cells
Codon
DNA
Genes, ras
Guanine
Molecular Sequence Data
Mutation
Rats

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