Article
8-Hydroxyguanine (7,8-dihydro-8-oxoguanine) in hot spots of the c-Ha-ras gene: effects of sequence contexts on mutation spectra.
Carcinogenesis - 1 Apr 1995
Kamiya H, Murata-Kamiya N, Koizume S, Inoue H, Nishimura S, Ohtsuka E
Abstract excerpt
We previously reported that 8-hydroxyguanine (7,8-dihydro-8-oxoguanine) at the second position of codon 12 of the c-Ha-ras gene induces many types of mutations in NIH3T3 cells. In this study we incorporated the modified base into the first and second positions of codon 12 in the coding strand and...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Base Sequence
- Codon
- DNA Mutational Analysis
- DNA Replication
- Gene Expression Regulation
- Genes, ras
- Genetic Vectors
- Guanine
- Mice
- Molecular Sequence Data
- Mutation
- Oligonucleotides
- Transfection
- Transformation, Genetic