Article
Mutations induced by DNA lesions in hot spots of the c-Ha-ras gene.
Nucleic acids symposium series - 1 Jan 1992
Kamiya H, Miura H, Suzuki M, Murata N, Ishikawa H, Shimizu M, Komatsu Y, Murata T, Sasaki T, Inoue H
Abstract excerpt
In order to investigate whether several DNA lesions (O6-methylguanine, 8-hydroxyguanine, xanthine, an abasic site analogue and hypoxanthine) activate a c-Ha-ras gene and to determine the type of mutations induced by the DNA lesions, they were introduced into a synthetic c-Ha-ras gene by DNA cassette mutagenesis techniques. The modified genes were transfected into mouse NIH3T3 cells and the c-Ha-ras genes present...
Topics
- 3T3 Cells
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Line, Transformed
- DNA
- DNA Damage
- Genes, ras
- Guanine
- Hypoxanthine
- Hypoxanthines
- Mice