Article
Ribozymes designed to inhibit transformation of NIH3T3 cells by the activated c-Ha-ras gene.
Gene - 15 Aug 1992
Koizumi M, Kamiya H, Ohtsuka E
Abstract excerpt
We have designed hammerhead ribozymes that cleave c-Ha-ras mRNA mutated at codon 12 (GGU----GUU). Plasmids containing the ribozyme-encoding genes were expressed under the control of the long terminal repeats of Rous sarcoma virus in NIH3T3 cells transfected with the activated c-Ha-ras gene. These...
Topics
- 3T3 Cells
- Animals
- Base Sequence
- Blotting, Northern
- Cell Transformation, Neoplastic
- Cloning, Molecular
- Gene Expression Regulation, Neoplastic
- Genes, ras
- Mice
- Molecular Sequence Data
- Mutation
- Nucleic Acid Conformation
- Plasmids
- Polymerase Chain Reaction
- RNA, Antisense
- RNA, Catalytic
- RNA, Messenger