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Article

Transforming activity of a synthetic c-Ha-ras gene containing O6-methylguanine in codon 12.

Japanese journal of cancer research : Gann - 1 Sept 1991

Kamiya H, Miura K, Ohtomo N, Nishimura S, Ohtsuka E

Abstract excerpt

A mutagenic DNA-adduct, O6-methylguanine, was introduced into codon 12 of the synthetic c-Ha-ras gene by cassette mutagenesis. Transfection of this modified ras gene into normal NIH3T3 cells by the calcium phosphate procedure resulted in significant induction of focus formation. The ras gene inserted into the transformed cells was found to have a G to A transition at the position of the modified base. These...

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Identifiers

PMID: 1938604
DOI: 10.1111/j.1349-7006.1991.tb01934.x
PMCID: PMC5918606

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Source receipt: pubmed, retrieved 7 Sept 2026.

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