Article
Transforming activity of a synthetic c-Ha-ras gene containing O6-methylguanine in codon 12.
Japanese journal of cancer research : Gann - 1 Sept 1991
Kamiya H, Miura K, Ohtomo N, Nishimura S, Ohtsuka E
Abstract excerpt
A mutagenic DNA-adduct, O6-methylguanine, was introduced into codon 12 of the synthetic c-Ha-ras gene by cassette mutagenesis. Transfection of this modified ras gene into normal NIH3T3 cells by the calcium phosphate procedure resulted in significant induction of focus formation. The ras gene inse...
Topics
- 3T3 Cells
- Adenine
- Amino Acid Sequence
- Animals
- Base Sequence
- Cell Transformation, Neoplastic
- Codon
- Genes, Synthetic
- Genes, ras
- Genetic Vectors
- Guanine
- Humans
- Mice
- Molecular Sequence Data
- Mutagenesis, Insertional
- Mutation
- Oligonucleotides
- Transfection