Article
In vivo and in vitro activities of point mutants of the bacteriophage T7 RNA polymerase promoter.
Biochemistry - 22 Sept 1992
Ikeda R A, Warshamana G S, Chang L L
Abstract excerpt
Two compatible plasmids were recently reported [Ikeda et al. (1992) Nucleic Acids Res. 20, 2517-2524] that together can be used to determine whether a mutant T7 RNA polymerase promoter is active or inactive in vivo. The first plasmid, pKGP1-1, carries T7 gene 1 (the gene encoding T7 RNA polymerase) ligated to a tac promoter, while the second plasmid, pCM-X#, carries the gene encoding chloramphenicol...
Topics
- Base Sequence
- Consensus Sequence
- DNA-Directed RNA Polymerases
- Molecular Sequence Data
- Mutation
- Promoter Regions, Genetic
- Structure-Activity Relationship
- Substrate Specificity
- T-Phages
- Transcription, Genetic
- Viral Proteins