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Article

Mutants with higher stability and specific activity from a single thermosensitive variant of T7 RNA polymerase.

Protein engineering, design & selection : PEDS - 1 Nov 2013

Boulain Jean-Claude, Dassa Janie, Mesta Laurent, Savatier Alexandra, Costa Narciso, Muller Bruno H, L'hostis Guillaume, Stura Enrico A, Troesch Alain, Ducancel Frédéric

Abstract excerpt

A single strategy to select RNA polymerase from bacteriophage T7 (T7 RNAP) mutants in Escherichia coli with enhanced thermostability or enzymatic activity is described. T7 RNAP has the ability to specifically transcribe genes under control of T7 phage promoter. By using random mutagenesis of the T7 RNAP gene in combination with an appropriate screening at 25 and 42°C, we have generated and selected E.coli clones...

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PMID: 24006372
DOI: 10.1093/protein/gzt040

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PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-08.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

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