Article
Correlation between transcription and C to T mutations in the non-transcribed DNA strand.
Biological chemistry - 1 Jan 2000
Beletskii A, Bhagwat A S
Abstract excerpt
We recently showed that transcription can promote C to T mutations in the non-transcribed strand in E. coli. To study the relationship between the level of transcription and mutant frequency, an inactive allele of the kanamycin-resistance gene was expressed under the control of a hybrid promoter consisting of an UP element and the tac promoter. When this promoter is induced, the frequency of C to T mutations in...
Topics
- Cell Line
- Cytosine
- DNA, Bacterial
- Escherichia coli
- Mutation
- Thymine
- Transcription, Genetic