Analysis of the effects of -42 and -32 ampC promoter mutations in clinical isolates of Escherichia coli hyperproducing ampC.

doi:10.1093/jac/45.6.783

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Article

Analysis of the effects of -42 and -32 ampC promoter mutations in clinical isolates of Escherichia coli hyperproducing ampC.

The Journal of antimicrobial chemotherapy - 1 Jun 2000

Caroff N, Espaze E, Gautreau D, Richet H, Reynaud A

Abstract excerpt

Escherichia coli usually produces only very small amounts of a constitutive AmpC beta-lactamase, but clinical strains overproducing this enzyme have been isolated. Three different ampC promoters of E. coli clinical strains were cloned upstream of the chloramphenicol acetyltransferase (CAT) gene in the pKK232-8 reporter plasmid and their relative strengths were compared by two different methods. The strength of...

Topics

Bacterial Proteins
Chloramphenicol O-Acetyltransferase
Escherichia coli
Microbial Sensitivity Tests
Mutagenesis, Site-Directed
Mutation
Plasmids
Promoter Regions, Genetic
Reverse Transcriptase Polymerase Chain Reaction
beta-Lactamases

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PMID: 10837430
DOI: 10.1093/jac/45.6.783

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