Article
Mutations in the chloramphenicol acetyltransferase (S61G, Y105C) increase accumulated amounts and resistance in Pseudomonas aeruginosa.
FEMS microbiology letters - 15 Jul 2004
Wang Jian, Liu Jian-Hua
Abstract excerpt
A chloramphenicol acetyltransferase (catB7) gene containing two point mutations, 181A/G and 314A/G, has been recently reported to be a determinant for high-level chloramphenicol resistance phenotype in a Pseudomonas aeruginosa strain PAhcr1. The mutant CATB7 was further characterized in vitro and in vivo to elucidate the molecular basis of high-level resistance. CAT assay demonstrated that the mutant and...
Topics
- Acetyl Coenzyme A
- Amino Acid Substitution
- Bacterial Proteins
- Chloramphenicol
- Chloramphenicol O-Acetyltransferase
- Chloramphenicol Resistance
- Cloning, Molecular
- Enzyme Stability
- Genes, Bacterial
- Microbial Sensitivity Tests