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Article

T7 promoter contacts essential for promoter activity in vivo.

Nucleic acids research - 25 May 1992

Ikeda R A, Ligman C M, Warshamana S

Abstract excerpt

T7 RNA polymerase promoters consist of a highly conserved 23 base-pair sequence that spans the site of the initiation of transcription (+1) and extends from -17 to +6. To determine the bases within the T7 consensus promoter that are essential for promoter function a library of mutant T7 promoters was constructed, and the in vivo activity of the mutant promoters was correlated to their sequence. The library of...

Topics

Base Sequence
Consensus Sequence
DNA-Directed RNA Polymerases
Escherichia coli
Molecular Sequence Data
Mutation
Oligodeoxyribonucleotides
Plasmids
Promoter Regions, Genetic
Recombinant Fusion Proteins
T-Phages

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Viral Proteins

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Identifiers

PMID: 1598210
DOI: 10.1093/nar/20.10.2517
PMCID: PMC312387

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 29 Aug 2026. Prepared record publication-1598210-2026-08-29.1. Manifest publication-graph-cohort-2026-08-29.1.

Source receipt: PubMed via Helena Literature Corpus, retrieved 29 Aug 2026.

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