Article
Genomic differences between O6-methylguanine-DNA methyltransferase proficient (Mex+) and deficient (Mex-) cell lines: possible role of genetic and epigenetic changes in conversion of Mex+ into Mex-.
Biochemical and biophysical research communications - 31 Mar 1992
Fritz G, Kaina B
Abstract excerpt
Cell lines that possess O6-methylguanine-DNA methyltransferase (MGMT) repair activity (Mex+ phenotype) or are deficient for MGMT (Mex-) are compared at genomic level. It is shown that 1) 208F rat cells do not contain the MGMT gene, as detected by Southern blot hybridization. 2) Mex- HeLa MR and CHO-9 cells express very low amounts of MGMT mRNA, as detected by PCR. The size of the MGMT specific PCR product was...
Topics
- Animals
- Cell Line
- Cell Survival
- HeLa Cells
- Humans
- Methylnitronitrosoguanidine
- Methyltransferases
- Mutagenesis
- Mutagens
- Mutation
- Nitrosourea Compounds