Nonrandom distribution of O6-methylguanine in H-ras gene sequence from DNA modified with N-methyl-N-nitrosourea.

doi:10.1016/0027-5107(93)90085-t

Back to search

Article

Nonrandom distribution of O6-methylguanine in H-ras gene sequence from DNA modified with N-methyl-N-nitrosourea.

Mutation research - 1 Aug 1993

Mironov N M, Bleicher F, Martel-Planche G, Montesano R

Abstract excerpt

The distribution of O6-meG in the rat H-ras gene sequence was studied using PCR by transition of O6-meG to adenine during the reaction. In order to study the transition mutations the PCR product was cloned in a replicative form of phage M13mp18 and sequenced. The use of PCR for detection of O6-me...

Topics

Animals
Base Sequence
DNA
Genes, ras
Guanine
Methylnitrosourea
Molecular Sequence Data
Mutation
Polymerase Chain Reaction
Rats

Community interpretation

Discuss the evidence

Ask a question, add context or challenge an interpretation. Community activity is separate from scientific evidence strength.

discussions

replies

Citations encouraged

Claims remain attributable.

No discussion yet

Be the first to add a careful, cited interpretation.