Article
Mouse methyltransferase for repair of O6-methylguanine and O4-methylthymine in DNA.
Carcinogenesis - 1 Jul 1995
Kawate H, Ihara K, Kohda K, Sakumi K, Sekiguchi M
Abstract excerpt
cDNA for mouse O6-methylguanine-DNA methyltransferase was expressed in methyltransferase-deficient Escherichia coli mutant cells, and the overproduced mouse enzyme was purified to a homogeneous state. Using this purified product, polyclonal antibodies were prepared and used to estimate amounts of the methyltransferase protein in cells. A single cell of NIH3T3 contained 1.8 x 10(4) molecules of the...
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