Article
Intrachromosomal probes for mutagenesis by alkylated DNA bases replicated in mammalian cells: a comparison of the mutagenicities of O4-methylthymine and O6-methylguanine in cells with different DNA repair backgrounds.
Chemical research in toxicology - 1 Sept 1996
Altshuler K B, Hodes C S, Essigmann J M
Abstract excerpt
A shuttle vector was constructed in which a single O4-methylthymine (O4MeThy) or O6-methylguanine (O6MeGua) was positioned within a unique NheI restriction site. These lesions are among the many produced when alkylating agents interact with DNA and are the two most widely believed to account for...
Topics
- Animals
- CHO Cells
- Cricetinae
- DNA
- DNA Repair
- Genetic Markers
- Genetic Vectors
- Guanine
- Methyltransferases
- Mutation
- O(6)-Methylguanine-DNA Methyltransferase
- Sequence Analysis
- Thymine
- Transfection