Article
Detection of p53 gene mutations in human ovarian and endometrial cancers by polymerase chain reaction-single strand conformation polymorphism analysis.
Japanese journal of cancer research : Gann - 1 Oct 1992
Naito M, Satake M, Sakai E, Hirano Y, Tsuchida N, Kanzaki H, Ito Y, Mori T
Abstract excerpt
The presence of mutations in the p53 gene was examined in ovarian cancers by a polymerase chain reaction-single strand conformation polymorphism (PCR-SSCP) analysis. The primers were designed to amplify exons 5 through 9 that contain phylogenetically conserved domains of the p53 gene. Mutations were detected in 5 out of 10 cases, one of which contained a deletion in the second allele. A single base substitution...
Topics
- Alleles
- Base Sequence
- Chromosome Deletion
- DNA
- DNA, Neoplasm
- DNA, Single-Stranded
- DNA, Viral
- Endometrial Neoplasms
- Exons
- Female
- Genes, p53