Article
Role of p53 mutations in endocrine tumorigenesis: mutation detection by polymerase chain reaction-single strand conformation polymorphism.
Cancer research - 15 Sept 1992
Yoshimoto K, Iwahana H, Fukuda A, Sano T, Saito S, Itakura M
Abstract excerpt
To elucidate the molecular basis for endocrine tumorigenesis, p53 mutations in human endocrine tumors were analyzed by using polymerase chain reaction-single strand conformation polymorphism. Exons 5 through 10 of the p53 gene were studied in genomic DNAs from 134 primary endocrine tumors and 6 human endocrine cancer-derived cell lines. Mutations were detected and identified in 4 endocrine tumors, including one...
Topics
- Base Sequence
- DNA, Neoplasm
- Electrophoresis, Polyacrylamide Gel
- Endocrine Gland Neoplasms
- Genes, p53
- Humans
- Molecular Sequence Data
- Mutation
- Oligodeoxyribonucleotides
- Polymerase Chain Reaction