Article
In vitro GAP activity towards RhoA, Rac1 and Cdc42 is not a prerequisite for YopE induced HeLa cell cytotoxicity.
Microbial pathogenesis - 1 Jun 2003
Aili Margareta, Telepnev Maxim, Hallberg Bengt, Wolf-Watz Hans, Rosqvist Roland
Abstract excerpt
The YopE cytotoxin of Yersinia is an essential virulence determinant that is translocated into the eukaryotic target cell via a plasmid-encoded type III secretion system. YopE possess a GTPase activating protein activity that in vitro has been shown to down regulate RhoA, Rac1, and Cdc42. Translocated YopE induces de-polymerisation of the actin microfilament structure in the eukaryotic cell which results in a...
Topics
- Amino Acid Sequence
- Bacterial Outer Membrane Proteins
- Bacterial Toxins
- Cytotoxicity Tests, Immunologic
- Cytotoxins
- GTPase-Activating Proteins
- Gene Deletion
- HeLa Cells
- Humans
- Models, Molecular
- Molecular Sequence Data
- Mutation
- Recombinant Proteins
- Sequence Alignment