Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Digital genotyping and haplotyping with polymerase colonies.

Proceedings of the National Academy of Sciences of the United States of America - 13 May 2003

Mitra Robi D, Butty Vincent L, Shendure Jay, Williams Benjamin R, Housman David E, Church George M

Abstract excerpt

Polymerase colony (polony) technology amplifies multiple individual DNA molecules within a thin acrylamide gel attached to a microscope slide. Each DNA molecule included in the reaction produces an immobilized colony of double-stranded DNA. We genotype these polonies by performing single base extensions with dye-labeled nucleotides, and we demonstrate the accurate quantitation of two allelic variants. We also...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to DNA
Share to DNA-Directed DNA Polymerase

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Gene Amplification

Share to Genetic Techniques

Share to Haplotypes

Share to Lod Score

Share to Models, Genetic

Share to Polymorphism, Single Nucleotide

Source

Open in PubMed Open full text

Identifiers

PMID: 12730373
DOI: 10.1073/pnas.0936399100
PMCID: PMC156303

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Digital genotyping and haplotyping with polymerase colonies.PMID 12730373Read ↗

Massively parallel haplotyping on microscopic beads for the high-throughput phase analysis of single molecules.Semantic proximity Read ↗

Improved multiple displacement amplification with phi29 DNA polymerase for genotyping of single human cells.Semantic proximity Read ↗

Haplotyping of Heterozygous SNPs in Genomic DNA Using Long-Range PCR.Semantic proximity Read ↗

Phasing DNA Markers Using Digital PCR.Semantic proximity Read ↗

High-throughput haplotype determination over long distances by haplotype fusion PCR and ligation haplotyping.Semantic proximity Read ↗

SNP genotyping and molecular haplotyping of DNA pools.Semantic proximity Read ↗

Genotyping SNPs with molecular beacons.Semantic proximity Read ↗

A rapid molecular approach for chromosomal phasing.Semantic proximity Read ↗

Whole-genome molecular haplotyping of single cells.Semantic proximity Read ↗

Clone-based systematic haplotyping (CSH): a procedure for physical haplotyping of whole genomes.Semantic proximity Read ↗

Genotyping and haplotyping of polymorphisms directly from genomic DNA via coupled amplification and sequencing (CAS).Semantic proximity Read ↗

In situ genotyping individual DNA molecules by target-primed rolling-circle amplification of padlock probes.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.