Discover Community Create You

Loading publication...

Noodle always counts identifier-free feature events for service operation. With your permission, Google Analytics can also estimate audience and engagement. Search text, publication identifiers, cookies, IP addresses and browser fingerprints are never included in Noodle's first-party event counters.

Privacy details

Article

Phasing DNA Markers Using Digital PCR.

Methods in molecular biology (Clifton, N.J.) - 1 Jan 2018

Regan John, Karlin-Neumann George

Abstract excerpt

Besides quantifying the absolute number of copies of known DNA targets, digital PCR can also be used to assess whether two nonpolymorphic gene sequences or two heterozygous markers reside on the same DNA molecule (i.e., are physically linked). Some useful linkage applications include: phasing variants to define a haplotype; genotyping of inversions; determining the presence of multimarker pathogenic bacteria in a...

Read the complete abstract on PubMed

Topics

Share this publication in a Topic to start or enrich a Post.

Share to Alleles
Share to Cystic Fibrosis Transmembrane Conductance Regulator
Share to DNA

Methodology Data sources Editorial policy Privacy About Contact Helena Bioinformatics

Share to Genetic Linkage

Share to Genetic Markers

Share to Haplotypes

Share to Polymerase Chain Reaction

Source

Identifiers

PMID: 29717461
DOI: 10.1007/978-1-4939-7778-9_28

Genes

CFTR

Provenance

PubMed metadata and Literature Corpus relationships. Corpus snapshot updated 7 Sept 2026. Manifest publication-graph-cohort-2026-09-07.1.

Source receipt: pubmed, retrieved 7 Sept 2026.

Related research

Semantic proximity does not establish scientific evidence.

Research trail→

Click a neighbor to travelStep 1 · 12 closest

Phasing DNA Markers Using Digital PCR.PMID 29717461Read ↗

Digital genotyping and haplotyping with polymerase colonies.Semantic proximity Read ↗

A rapid molecular approach for chromosomal phasing.Semantic proximity Read ↗

The accuracy of heterozygous base calling from diploid sequence and resolution of haplotypes using allele-specific sequencing.Semantic proximity Read ↗

Development and use of molecular markers: past and present.Semantic proximity Read ↗

PhaseME: Automatic rapid assessment of phasing quality and phasing improvement.Semantic proximity Read ↗

DNA Sudoku--harnessing high-throughput sequencing for multiplexed specimen analysis.Semantic proximity Read ↗

Haplotyping RAD loci: an efficient method to filter paralogs and account for physical linkage.Semantic proximity Read ↗

The linkage method: a novel approach for SNP detection and haplotype reconstruction from a single diploid individual using next-generation sequence data.Semantic proximity Read ↗

Targeted Locus Amplification and Haplotyping.Semantic proximity Read ↗

Allelic discrimination of cis-trans relationships by digital polymerase chain reaction: GJB2 (p.V27I/p.E114G) and CFTR (p.R117H/5T).Semantic proximity Read ↗

Identification of SNPs, or mutations in sequence chromatograms.Semantic proximity Read ↗

Novel Multiplexing Strategies for Quantification of Rare Alleles Using ddPCR.Semantic proximity Read ↗

⌁Select a neighboring publication to make it the new centre.