Improved multiple displacement amplification with phi29 DNA polymerase for genotyping of single human cells.

doi:10.2144/000112755

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Article

Improved multiple displacement amplification with phi29 DNA polymerase for genotyping of single human cells.

BioTechniques - 1 Jun 2008

Kumar Gyanendra, Garnova Elena, Reagin Michael, Vidali Andrea

Abstract excerpt

The ability to genotype multiple loci of single cells would be of significant benefit to investigations of cellular processes such as oncogenesis, meiosis, fertilization, and embryogenesis. We report a simple two-step, single-tube protocol for whole-genome amplification (WGA) from single human cells using components of the GenomiPhi V2 DNA Amplification kit. For the first time, we demonstrate reliable generation...

Topics

Bacillus Phages
Genotype
Humans
Microsatellite Repeats
Minisatellite Repeats
Nucleic Acid Amplification Techniques
Polymorphism, Single Nucleotide
Reagent Kits, Diagnostic

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PMID: 18533898
DOI: 10.2144/000112755

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