Genotyping and haplotyping of polymorphisms directly from genomic DNA via coupled amplification and sequencing (CAS).

doi:10.1093/nar/19.24.6877

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Article

Genotyping and haplotyping of polymorphisms directly from genomic DNA via coupled amplification and sequencing (CAS).

Nucleic acids research - 25 Dec 1991

Ruano G, Kidd K K

Abstract excerpt

Coupled amplification and sequencing (CAS) allows a segment of DNA to be sequenced directly from genomic DNA. An initial PCR amplification stage selects and amplifies the target. During a subsequent stage both strands of the target segment are sequenced simultaneously and amplified further. We sh...

Topics

Animals
Base Sequence
DNA
Electrophoresis
Female
Genes, Homeobox
Genetic Testing
Genome
Genotype
Globins
Haplotypes
Humans
Male
Molecular Sequence Data
Multigene Family
Oligodeoxyribonucleotides
Pan troglodytes
Polymerase Chain Reaction

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