Article
Genotyping and haplotyping of polymorphisms directly from genomic DNA via coupled amplification and sequencing (CAS).
Nucleic acids research - 25 Dec 1991
Ruano G, Kidd K K
Abstract excerpt
Coupled amplification and sequencing (CAS) allows a segment of DNA to be sequenced directly from genomic DNA. An initial PCR amplification stage selects and amplifies the target. During a subsequent stage both strands of the target segment are sequenced simultaneously and amplified further. We show that CAS can readily identify variant base pairs. Genotyping of a population for known sequence variation can be...
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